6FD9

Catalytic subunit HisG from Psychrobacter arcticus ATP phosphoribosyltransferase (HisZG ATPPRT) in complex with AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-06
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.5418
Spacegroup name	I 1 2 1
Unit cell lengths	70.820, 33.940, 92.407
Unit cell angles	90.00, 105.36, 90.00

Refinement procedure

Resolution	26.610 - 2.200
R-factor	0.1874
Rwork	0.185
R-free	0.23270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5M8H chain E
RMSD bond length	0.008
RMSD bond angle	1.308
Data reduction software	MOSFLM
Data scaling software	Aimless (0.5.32)
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.610	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.168	0.549
Rmeas	0.199	0.645
Rpim	0.105	0.336
Total number of observations	37047
Number of reflections	10299	827
<I/σ(I)>	5.8
Completeness [%]	93.0	87.9
Redundancy	3.6	3.6
CC(1/2)	0.981	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	277	10mg/ml protein in 20mM Tris HCl pH8.0, 50mM KCl, 10mM MgCl2, 2mM DTT mixed 1:1 with 32% PEG 3350, 0.1M MOPS pH6.5, 0.1M K/Na tartrate