6FD8

Gamma-s crystallin dimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2017-07-15
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9763
Spacegroup name	P 1
Unit cell lengths	49.632, 52.365, 53.972
Unit cell angles	108.72, 111.46, 105.50

Refinement procedure

Resolution	43.114 - 2.100
R-factor	0.2283
Rwork	0.226
R-free	0.27310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vh1
RMSD bond length	0.008
RMSD bond angle	0.943
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.114	2.211
High resolution limit [Å]	2.100	2.100
Rmerge	0.032
Rpim	0.022
Number of reflections	23548	2372
<I/σ(I)>	7.8
Completeness [%]	93.6	94.35
Redundancy	2.6
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.2 M Sodium tartrate dibasic dihydrate (pH 7.3), 20% w/v polyethylene glycol 3350