6FCY
Catalytic subunit HisG from Psychrobacter arcticus ATP phosphoribosyltransferase (HisZG ATPPRT) in complex with PRPP and ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-08 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 70.580, 34.040, 93.868 |
Unit cell angles | 90.00, 91.61, 90.00 |
Refinement procedure
Resolution | 23.030 - 1.960 |
R-factor | 0.1864 |
Rwork | 0.185 |
R-free | 0.22280 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5M8H chain E |
RMSD bond length | 0.007 |
RMSD bond angle | 1.383 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.5.32) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.030 | 2.010 |
High resolution limit [Å] | 1.960 | 1.960 |
Rmerge | 0.094 | 0.477 |
Rmeas | 0.109 | 0.583 |
Rpim | 0.054 | 0.325 |
Total number of observations | 63590 | |
Number of reflections | 16164 | 1029 |
<I/σ(I)> | 9 | |
Completeness [%] | 99.1 | 90.1 |
Redundancy | 3.9 | 3 |
CC(1/2) | 0.996 | 0.762 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 277 | 10mg/ml protein in 20mM Tris HCl pH8.0, 50mM KCl, 10mM MgCl2, 2mM DTT mixed 1:1 with 32% PEG 3350, 0.1M MOPS pH6.5, 0.1M K/Na tartrate |