6FCW
Catalytic subunit HisG from Psychrobacter arcticus ATP phosphoribosyltransferase (HisZG ATPPRT) in complex with PRATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-05-10 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5148 |
Spacegroup name | I 1 2 1 |
Unit cell lengths | 70.700, 33.940, 91.437 |
Unit cell angles | 90.00, 104.76, 90.00 |
Refinement procedure
Resolution | 23.820 - 2.000 |
R-factor | 0.1938 |
Rwork | 0.192 |
R-free | 0.23150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Chain E of 5M8H |
RMSD bond length | 0.008 |
RMSD bond angle | 1.372 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.5.32) |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.820 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.172 | 0.621 |
Rmeas | 0.193 | 0.704 |
Rpim | 0.087 | 0.325 |
Number of reflections | 13619 | 915 |
<I/σ(I)> | 7 | |
Completeness [%] | 93.9 | 88.1 |
Redundancy | 4.7 | 4.3 |
CC(1/2) | 0.989 | 0.720 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 6.5 | 277 | 10mg/ml protein in 20mM Tris HCl pH8.0, 50mM KCl, 10mM MgCl2, 2mM DTT mixed 1:1 with 32% PEG 3350, 0.1M MOPS pH6.5, 0.1M K/Na tartrate |