6FCM
Crystal structure of human PCNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-10-29 |
Detector | DECTRIS PILATUS3 X 2M |
Wavelength(s) | 0.96600 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.180, 140.060, 169.970 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.000 - 2.800 |
R-factor | 0.19917 |
Rwork | 0.195 |
R-free | 0.27588 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1axc |
RMSD bond length | 0.008 |
RMSD bond angle | 1.310 |
Data reduction software | XDS (VERSION May 1, 2016 BUILT=20160617) |
Data scaling software | XSCALE (VERSION May 1, 2016 BUILT=20160617) |
Phasing software | PHASER (2.6.1) |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.000 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.048 | 0.802 |
Rmeas | 0.056 | 0.962 |
Number of reflections | 23289 | 1567 |
<I/σ(I)> | 18.27 | |
Completeness [%] | 97.0 | 92.2 |
Redundancy | 3.43 | 3.03 |
CC(1/2) | 0.999 | 0.719 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 19 to 23% PEG 3350, 0.2 to 0.3 M NaCl, 0.1 M Tris-HCl pH 8.5 |