6FCD
Human Methionine Adenosyltransferase II mutant (R264A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-09-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 67.141, 94.230, 116.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.410 - 1.700 |
| R-factor | 0.16116 |
| Rwork | 0.160 |
| R-free | 0.18423 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a1i |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.695 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.410 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.124 | 0.513 |
| Rmeas | 0.158 | 0.665 |
| Rpim | 0.096 | 0.419 |
| Number of reflections | 40576 | 2138 |
| <I/σ(I)> | 5.9 | 4.1 |
| Completeness [%] | 99.0 | 99.8 |
| Redundancy | 4.3 | 4.1 |
| CC(1/2) | 0.985 | 0.619 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 30% PEG600 0.1M HEPES pH 7.0 |
