6FCC
Catalytic subunit HisG from Psychrobacter arcticus ATP phosphoribosyltransferase (HisZG ATPPRT)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-24 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 70.980, 33.870, 92.367 |
| Unit cell angles | 90.00, 105.92, 90.00 |
Refinement procedure
| Resolution | 21.500 - 1.890 |
| R-factor | 0.2086 |
| Rwork | 0.207 |
| R-free | 0.24530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5M8H chain E |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.292 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.500 | 1.930 |
| High resolution limit [Å] | 1.890 | 1.890 |
| Rmerge | 0.120 | 0.460 |
| Rmeas | 0.160 | 0.600 |
| Rpim | 0.100 | 0.380 |
| Number of reflections | 17009 | 861 |
| <I/σ(I)> | 6.9 | 1.9 |
| Completeness [%] | 98.1 | 79.3 |
| Redundancy | 4.4 | 3.6 |
| CC(1/2) | 0.990 | 0.860 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 277 | 10mg/ml-1 protein (buffer 20mM Tris HCl pH8.0, 50mM KCl, 10mM MgCl2, 2mM DTT) mixed 1:1 with 32% PEG 3350, 0.1M MOPS pH6.5, 0.1M K/Na tartrate |
