6FBX

Crystal Structure of a Zebra-fish pro-survival protein NRZ:Bad BH3 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95373
Spacegroup name	P 63
Unit cell lengths	87.619, 87.619, 36.770
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.810 - 1.639
R-factor	0.1889
Rwork	0.188
R-free	0.20670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ku9
RMSD bond length	0.003
RMSD bond angle	0.506
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.810	1.680
High resolution limit [Å]	1.639	1.640
Rmerge	0.090	1.920
Rmeas	0.095
Rpim	0.030
Number of reflections	20018	1467
<I/σ(I)>	15.4	1.3
Completeness [%]	100.0	100
Redundancy	18.6	9.9
CC(1/2)	1.000	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	20% PEG3350, 0.2M Sodium fluoride, 0.1M Bis-Tris propane