6FBT
The X-ray Structure of Lytic Transglycosylase Slt from Pseudomonas aeruginosa in complex with the reaction product NAG-anhNAMpentapeptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALBA BEAMLINE XALOC |
Synchrotron site | ALBA |
Beamline | XALOC |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97919 |
Spacegroup name | P 63 |
Unit cell lengths | 165.526, 165.526, 54.733 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 143.350 - 2.500 |
R-factor | 0.18298 |
Rwork | 0.180 |
R-free | 0.23944 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ohu |
RMSD bond length | 0.013 |
RMSD bond angle | 1.673 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 143.350 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Rpim | 0.024 | 0.360 |
Number of reflections | 30002 | |
<I/σ(I)> | 15.2 | |
Completeness [%] | 99.9 | |
Redundancy | 15.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 1.4M sodium potassium phosphate pH 6.5 |