6FBO
Human Methionine Adenosyltransferase II mutant (S114A) in I222 crystal form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 66.621, 94.682, 116.550 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.280 - 1.800 |
| R-factor | 0.15215 |
| Rwork | 0.151 |
| R-free | 0.17436 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5a1i |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.671 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.280 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.170 | 0.766 |
| Rmeas | 0.208 | 0.940 |
| Rpim | 0.119 | 0.538 |
| Number of reflections | 34507 | 2037 |
| <I/σ(I)> | 5.8 | |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.3 | 5.4 |
| CC(1/2) | 0.979 | 0.782 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 30% PEG600 0.1M HEPES pH.7.0 |
