6FBK
Crystal structure of the human WNK2 CCT-like 1 domain in complex with a WNK1 RFXV peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-30 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | I 41 |
| Unit cell lengths | 78.047, 78.047, 37.736 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.973 - 1.743 |
| R-factor | 0.2869 |
| Rwork | 0.284 |
| R-free | 0.33410 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6elm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.874 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.300 | 1.773 |
| High resolution limit [Å] | 1.740 | 1.743 |
| Number of reflections | 11689 | |
| <I/σ(I)> | 20.7 | |
| Completeness [%] | 99.3 | |
| Redundancy | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 20% PEG3350 -- 10% ethylene glycol -- 0.2M sodium fluoride |
