6FBK
Crystal structure of the human WNK2 CCT-like 1 domain in complex with a WNK1 RFXV peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-30 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | I 41 |
Unit cell lengths | 78.047, 78.047, 37.736 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.973 - 1.743 |
R-factor | 0.2869 |
Rwork | 0.284 |
R-free | 0.33410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6elm |
RMSD bond length | 0.008 |
RMSD bond angle | 0.874 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.300 | 1.773 |
High resolution limit [Å] | 1.740 | 1.743 |
Number of reflections | 11689 | |
<I/σ(I)> | 20.7 | |
Completeness [%] | 99.3 | |
Redundancy | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 20% PEG3350 -- 10% ethylene glycol -- 0.2M sodium fluoride |