6FBH
KlenTaq DNA polymerase processing a modified primer - bearing the modification upstream at the sixth primer nucleotide.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-06-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00002 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 109.706, 109.706, 91.292 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.504 - 1.800 |
| R-factor | 0.1975 |
| Rwork | 0.196 |
| R-free | 0.22300 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.841 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12rc1_2815: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.504 | 1.809 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmeas | 0.099 | 1.862 |
| Number of reflections | 113900 | 18357 |
| <I/σ(I)> | 8.68 | 0.67 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 5.14 | 5.02 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 291 | 29 % PEG 4000, 0.05 M TRIS, 20 mM manganese(II) chloride, 0.2 M ammonium chloride |
