6FBH

KlenTaq DNA polymerase processing a modified primer - bearing the modification upstream at the sixth primer nucleotide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00002
Spacegroup name	P 31 2 1
Unit cell lengths	109.706, 109.706, 91.292
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.504 - 1.800
R-factor	0.1975
Rwork	0.196
R-free	0.22300
RMSD bond length	0.009
RMSD bond angle	0.841
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.12rc1_2815: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.504	1.809
High resolution limit [Å]	1.800	1.800
Rmeas	0.099	1.862
Number of reflections	113900	18357
<I/σ(I)>	8.68	0.67
Completeness [%]	99.8	99.7
Redundancy	5.14	5.02

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	29 % PEG 4000, 0.05 M TRIS, 20 mM manganese(II) chloride, 0.2 M ammonium chloride