6FBD

KlenTaq DNA polymerase processing a modified primer - bearing the modification upstream at the second primer nucleotide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.000000
Spacegroup name	P 31 2 1
Unit cell lengths	109.964, 109.964, 91.165
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.082 - 2.099
R-factor	0.2001
Rwork	0.198
R-free	0.23310
RMSD bond length	0.007
RMSD bond angle	0.575
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.12rc1_2815: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.616	2.110
High resolution limit [Å]	2.099	2.099
Rmeas	0.073	0.873
Number of reflections	72040	11669
<I/σ(I)>	13.91	1.94
Completeness [%]	99.9	99.8
Redundancy	5.18	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	291	16 % PEG 4000, 0.1 M HEPES, 25 mM manganese(II) chloride, 0.1 M sodium acetate