6FBC
KlenTaq DNA polymerase processing a modified primer - bearing the modification at the 3'-terminus of the primer.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-08-22 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.00004 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 109.230, 109.230, 90.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.298 - 1.540 |
| R-factor | 0.187 |
| Rwork | 0.186 |
| R-free | 0.20850 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.842 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12rc1_2815: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.298 | 1.548 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmeas | 0.092 | 2.360 |
| Number of reflections | 172578 | 28726 |
| <I/σ(I)> | 10.89 | |
| Completeness [%] | 96.3 | 99.1 |
| Redundancy | 5.05 | 4.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 291 | 14 % PEG 4000, 0.1 M HEPES, 20 mM manganese(II) chloride, 0.1 M sodium actetate |
