6FB4
human KIBRA C2 domain mutant C771A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-09-03 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 83.880, 83.880, 207.770 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 72.642 - 2.416 |
| R-factor | 0.203125005734 |
| Rwork | 0.201 |
| R-free | 0.24746 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k9d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | BALBES |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.643 | 2.500 |
| High resolution limit [Å] | 2.415 | 2.420 |
| Rmerge | 0.133 | 0.625 |
| Rmeas | 0.140 | 0.676 |
| Number of reflections | 17355 | |
| <I/σ(I)> | 10.7 | 1.7 |
| Completeness [%] | 99.9 | 98.6 |
| Redundancy | 10.6 | 6.22 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.1M Na HEPES, 0.8M Na dihydrogen phosphare, 0.8M K dihydrogen phosphate 5% glycerol cryoprotectant |
