6FAQ
Structure of H. salinarum RosR (vng0258) grown from KBr
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-15 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.91508 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.149, 76.406, 42.098 |
| Unit cell angles | 90.00, 109.47, 90.00 |
Refinement procedure
| Resolution | 38.200 - 1.950 |
| R-factor | 0.1908 |
| Rwork | 0.189 |
| R-free | 0.23580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SAD DERIVED MODEL IN NACL |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.356 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.690 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.104 | 0.950 |
| Rmeas | 0.112 | 0.880 |
| Rpim | 0.041 | 0.730 |
| Number of reflections | 33425 | 5717 |
| <I/σ(I)> | 12.9 | 1.59 |
| Completeness [%] | 94.1 | 98.7 |
| Redundancy | 7.5 | 3.85 |
| CC(1/2) | 0.998 | 0.797 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | KBR, MES, AMMONIUM SULFATE, PH 6.8, EVAPORATION, TEMPERATURE 298K |
