6FAJ

The structure of Human Methionine Adenosyltransferase II in apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	P 2 21 21
Unit cell lengths	62.176, 103.232, 108.359
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	74.740 - 1.950
R-factor	0.19146
Rwork	0.190
R-free	0.22387
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5a1i
RMSD bond length	0.013
RMSD bond angle	1.666
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.740	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.181	0.387
Rmeas	0.236	0.506
Rpim	0.150	0.322
Number of reflections	51446	3573
<I/σ(I)>	4.7	2.1
Completeness [%]	99.6	99.8
Redundancy	3.5	3.6
CC(1/2)	0.940	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	35% PEG600 0.1M HEPES pH7.0