6FAJ
The structure of Human Methionine Adenosyltransferase II in apo state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-06-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 62.176, 103.232, 108.359 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 74.740 - 1.950 |
R-factor | 0.19146 |
Rwork | 0.190 |
R-free | 0.22387 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5a1i |
RMSD bond length | 0.013 |
RMSD bond angle | 1.666 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.740 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.181 | 0.387 |
Rmeas | 0.236 | 0.506 |
Rpim | 0.150 | 0.322 |
Number of reflections | 51446 | 3573 |
<I/σ(I)> | 4.7 | 2.1 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 3.5 | 3.6 |
CC(1/2) | 0.940 | 0.845 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 35% PEG600 0.1M HEPES pH7.0 |