6FAA

CRYSTAL STRUCTURE OF THE DEAH-BOX HELICASE PRP2 IN COMPLEX WITH ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-01-23
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9202
Spacegroup name	P 21 21 21
Unit cell lengths	50.260, 114.210, 117.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.003 - 1.970
R-factor	0.1894
Rwork	0.188
R-free	0.22420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2xau
RMSD bond length	0.005
RMSD bond angle	1.051
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.070
High resolution limit [Å]	1.970	1.970
Rmerge	0.092	0.846
Rmeas	0.107
Number of reflections	48358
<I/σ(I)>	13.43	1.77
Completeness [%]	99.0	99.3
Redundancy	3.74
CC(1/2)	0.997

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.20 M NaCl, 15% (w/v) PEG8000, 0.1 M Tris/HCl pH 7.5