6FA9

CRYSTAL STRUCTURE OF THE DEAH-BOX HELICASE PRP2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-06-07
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	50.610, 114.130, 120.690
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.462 - 2.600
R-factor	0.2323
Rwork	0.230
R-free	0.27590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6faa
RMSD bond length	0.004
RMSD bond angle	0.852
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	41.462	50.000	2.700
High resolution limit [Å]	2.600	41.462	2.600
Rmerge	0.070		0.840
Rmeas	0.078		0.936
Number of reflections	22177		2304
<I/σ(I)>	20.43		2.34
Completeness [%]	99.8		99.9
Redundancy	4.861		4.983
CC(1/2)	0.999		0.825

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	0.1 M Tris/HCl pH 7.5, 0.20 M Li2SO4, 15% (w/v) PEG 8000