6FA0
Lysozyme crystallized in presence of 100 mM ammonium sulphate at pH 4.5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-24 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.966 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 30.088, 56.282, 73.208 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.788 - 1.300 |
R-factor | 0.153 |
Rwork | 0.151 |
R-free | 0.18760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4e3u |
RMSD bond length | 0.007 |
RMSD bond angle | 0.884 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 19.790 | 19.790 | 1.320 |
High resolution limit [Å] | 1.300 | 7.120 | 1.300 |
Rmerge | 0.048 | 0.035 | 0.697 |
Rmeas | 0.054 | 0.039 | 0.788 |
Rpim | 0.024 | 0.018 | 0.359 |
Number of reflections | 28509 | 220 | 1504 |
<I/σ(I)> | 12.9 | ||
Completeness [%] | 91.0 | 94.2 | 99.6 |
Redundancy | 4.3 | 4.1 | 4.6 |
CC(1/2) | 0.999 | 0.999 | 0.747 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 100 mM Ammonium Sulphate, 0.4 M Sodium Chloride, 0.1 M Sodium Acetate |