6F9Z
Lysozyme crystallized in presence of 5 mM ammonium sulphate at pH 4.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-24 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.009, 56.132, 72.193 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.719 - 1.200 |
| R-factor | 0.1574 |
| Rwork | 0.156 |
| R-free | 0.17770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e3u |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.142 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.720 | 19.720 | 1.220 |
| High resolution limit [Å] | 1.200 | 6.460 | 1.200 |
| Rmerge | 0.036 | 0.022 | 0.454 |
| Rmeas | 0.041 | 0.025 | 0.529 |
| Rpim | 0.020 | 0.012 | 0.267 |
| Total number of observations | 146195 | ||
| Number of reflections | 37901 | 286 | 1910 |
| <I/σ(I)> | 15.5 | ||
| Completeness [%] | 97.5 | 95.1 | 97.9 |
| Redundancy | 3.9 | 3.6 | 3.6 |
| CC(1/2) | 0.999 | 0.999 | 0.803 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 5 mM Ammonium Sulphate, 0.4 M Sodium Chloride, 0.1 M Sodium Acetate |
