6F9X
Lysozyme crystallized in presence of 100 mM lithium sulphate at pH 4.5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-07-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.090, 56.214, 73.134 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.775 - 1.250 |
| R-factor | 0.1669 |
| Rwork | 0.165 |
| R-free | 0.19400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e3u |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.879 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.770 | 19.770 | 1.270 |
| High resolution limit [Å] | 1.250 | 6.850 | 1.250 |
| Rmerge | 0.057 | 0.038 | 0.577 |
| Rmeas | 0.064 | 0.043 | 0.645 |
| Rpim | 0.028 | 0.020 | 0.282 |
| Total number of observations | 155400 | 1162 | 3630 |
| Number of reflections | 30557 | 255 | 752 |
| <I/σ(I)> | 12.8 | 28.3 | 2.3 |
| Completeness [%] | 86.9 | 95.6 | 45.2 |
| Redundancy | 5.1 | 4.6 | 4.8 |
| CC(1/2) | 0.996 | 0.994 | 0.849 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 298 | 100 mM Lithium Sulphate, 0.3 M Sodium Chloride, 0.1 M Sodium Acetate |
