6F9W
Crystal structure of the LSM domain of LSM14 in complex with a C-terminal peptide of 4E-T
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-11 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.979090 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 64.890, 64.890, 61.670 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.884 - 2.623 |
| R-factor | 0.2218 |
| Rwork | 0.221 |
| R-free | 0.24530 |
| Structure solution method | SAD |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.732 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.884 | 2.720 |
| High resolution limit [Å] | 2.623 | 2.623 |
| Rmerge | 0.090 | 0.657 |
| Number of reflections | 4287 | 418 |
| <I/σ(I)> | 54.4 | 8.576 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 49.6 | 50.1 |
| CC(1/2) | 1.000 | 0.982 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 2 M (NH4)2SO4, 2 % PEG400, 0.1 M Hepes pH 7 |
