6F9S
Crystal structure of the C-terminal RecA domain of DDX6 in complex with a conserved peptide from LSM14
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-09 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.979340 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 92.148, 92.148, 149.896 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.070 - 3.030 |
R-factor | 0.2061 |
Rwork | 0.204 |
R-free | 0.24230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2way |
RMSD bond length | 0.003 |
RMSD bond angle | 0.484 |
Data reduction software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.070 | 3.140 |
High resolution limit [Å] | 3.030 | 3.030 |
Rmerge | 0.108 | 1.118 |
Number of reflections | 7824 | 763 |
<I/σ(I)> | 31.5 | 3.5 |
Completeness [%] | 99.8 | 99.4 |
Redundancy | 18.9 | 19.7 |
CC(1/2) | 0.999 | 0.875 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 1.55 M Li2SO4, 0.1 M Hepes pH 7.5 |