6F9N

CRYSTAL STRUCTURE OF THE HUMAN CPSF160-WDR33 COMPLEX

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-05
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00000
Spacegroup name	P 1
Unit cell lengths	67.910, 77.400, 104.020
Unit cell angles	87.56, 76.41, 67.00

Refinement procedure

Resolution	47.312 - 2.500
R-factor	0.2292
Rwork	0.228
R-free	0.26270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ei1
RMSD bond length	0.002
RMSD bond angle	0.555
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.312	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.090	0.773
Number of reflections	65410	6519
<I/σ(I)>	18.3	2.5
Completeness [%]	100.0	100
Redundancy	7.5	5.4
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	11% w/v PEG3400 37.5 mM Ammonium Formate 112.5 mM Magnesium Formate