6F9I
Crystal structure of KLC2 bound to the second tryptophan-acidic motif peptide from calsyntenin-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-12-18 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.92 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 75.442, 75.442, 303.356 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.458 - 3.993 |
R-factor | 0.254 |
Rwork | 0.253 |
R-free | 0.27340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zfw |
RMSD bond length | 0.005 |
RMSD bond angle | 0.976 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.460 | 4.210 |
High resolution limit [Å] | 3.990 | 3.990 |
Number of reflections | 8641 | |
<I/σ(I)> | 6.28 | |
Completeness [%] | 94.9 | |
Redundancy | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 298 | 0.893 M sodium potassium tartrate, 0.2 M NaCl, 0.1 M imidazole pH 8.0 |