6F8L
Thermus thermophilus PilF ATPase (AMPPNP-bound form)
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | k2 |
| Detector | GATAN K2 BASE (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 8 |
| Software | EMAN,RELION,EPU |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
