6F8G
Co-crystal structure of SPOP MATH domain and hamster Pdx1 fragment
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-11 |
| Detector | DECTRIS PILATUS3 X 2M |
| Wavelength(s) | 0.8726 |
| Spacegroup name | P 1 |
| Unit cell lengths | 45.400, 55.170, 58.070 |
| Unit cell angles | 89.25, 89.89, 86.76 |
Refinement procedure
| Resolution | 19.850 - 2.030 |
| R-factor | 0.18806 |
| Rwork | 0.184 |
| R-free | 0.26169 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ivv |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.693 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.102 |
| High resolution limit [Å] | 2.030 | 2.050 |
| Rmeas | 0.106 | |
| Number of reflections | 32906 | |
| <I/σ(I)> | 8.62 | |
| Completeness [%] | 96.4 | |
| Redundancy | 3.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 0.1 M Imidazole-HCl pH 8.0, 30% (w/v) MPD, 10% (w/v) PEG-4000 |
