6F8F
Co-crystal structure of SPOP MATH domain and human Pdx1 fragment
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.82655 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 53.925, 53.925, 207.410 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.940 - 2.000 |
| R-factor | 0.21841 |
| Rwork | 0.215 |
| R-free | 0.27433 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ivv |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.830 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.051 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.039 | |
| Number of reflections | 13193 | |
| <I/σ(I)> | 26.23 | |
| Completeness [%] | 99.0 | |
| Redundancy | 8.96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 0.1 M HEPES pH 6.5, 10% (w/v) PEG6000 (final pH 7.0) |
