6F8D

Crystal structure of Human ARS2 residues 171-270 + 408-763 (P65 form)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-14
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.984
Spacegroup name	P 65
Unit cell lengths	105.210, 105.210, 267.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	91.110 - 3.480
R-factor	0.26537
Rwork	0.264
R-free	0.29224
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f7j
RMSD bond length	0.007
RMSD bond angle	0.962
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.110	3.570
High resolution limit [Å]	3.480	3.480
Number of reflections	20970
<I/σ(I)>	8.71	0.8
Completeness [%]	97.9	86.6
Redundancy	3.12	2.98
CC(1/2)	0.997	0.253

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	10.5	277	Crystals of ARS2 were obtained at 4 C in 2 microlitre hanging drops with a 1:1 ratio of protein solution (6 mg/ml in 20 mM HEPES, 300 mM NaCl, 2 mM tris(2-carboxyethyl)phosphine pH 7.8) to crystallisation solution. The crystallisation solution was 1.2 M sodium dihydrogen phosphate, 0.8 M dipotassium hydrogen phosphate, 0.2 M lithium sulphate, 0.1 M CAPS pH 10.5