6F7P

Crystal structure of Human ARS2 residues 147-270 + 408-763

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-1
Synchrotron site	ESRF
Beamline	MASSIF-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-31
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.966
Spacegroup name	P 31 2 1
Unit cell lengths	136.640, 136.640, 158.790
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	118.330 - 3.700
R-factor	0.27606
Rwork	0.274
R-free	0.30909
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f7j
RMSD bond length	0.007
RMSD bond angle	1.014
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	118.330	3.800
High resolution limit [Å]	3.700	3.700
Number of reflections	18658
<I/σ(I)>	12.11	1.44
Completeness [%]	99.7	98.2
Redundancy	6.33	6.16
CC(1/2)	1.000	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	281	Crystals were obtained at 4 C in 2 microlitre hanging drops with a 1:1 ratio of protein solution at 6 mg per ml in 20 mM HEPES, 300 mM NaCl, 2 mM tris(2-carboxyethyl)phosphine pH 7.8) to crystallisation solution. The crystallisation solution was 0.2 M lithium sulphate and 20% (w/v) PEG 3550.