6F7K

Crystal structure of Dettilon tailspike protein (gp208)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9184
Spacegroup name	P 21 21 21
Unit cell lengths	153.422, 160.827, 161.717
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.760 - 2.100
R-factor	0.18312
Rwork	0.183
R-free	0.22068
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f7d
RMSD bond length	0.012
RMSD bond angle	1.225
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.760	2.230
High resolution limit [Å]	2.100	2.100
Rmeas	0.017	0.229
Number of reflections	231572	36799
<I/σ(I)>	10.6	0.78
Completeness [%]	99.5	98.7
Redundancy	6.72
CC(1/2)	0.998	0.452

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.85M Succinic acid pH 7.0, 0.1 M HEPES pH 7.0, 1 %(w/v) PEG 2000 MME