6F7J

Crystal structure of Human ARS2 residues 171-270 + 408-763

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.984
Spacegroup name	P 65 2 2
Unit cell lengths	90.920, 90.920, 267.020
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	78.740 - 3.220
R-factor	0.29033
Rwork	0.289
R-free	0.32174
Structure solution method	SIRAS
RMSD bond length	0.007
RMSD bond angle	0.937
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	CRANK2
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.740	3.300
High resolution limit [Å]	3.220	3.220
Number of reflections	10951	805
<I/σ(I)>	10.15	1.42
Completeness [%]	97.0	99.9
Redundancy	10.13	9.13
CC(1/2)	0.996	0.544

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	10.5	281	Protein solution at 6 mg per ml in 20 mM HEPES, 300 mM NaCl, 2 mM tris(2-carboxyethyl)phosphine pH 7.8, mixed 1:1 with reservoir solution containing 1.2 M sodium dihydrogen phosphate, 0.8 M dipotassium hydrogen phosphate, 0.2 M lithium sulphate and 0.1 M CAPS pH 10.5.