6F7D

Crystal structure of Dettilon tailspike protein (gp208)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-04-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.91841, 1.009, 1.072
Spacegroup name	I 21 3
Unit cell lengths	151.623, 151.623, 151.623
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.523 - 1.634
R-factor	0.16
Rwork	0.159
R-free	0.18770
RMSD bond length	0.011
RMSD bond angle	1.126
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.11.1-2575_2579: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.950	1.730
High resolution limit [Å]	1.630	1.630
Rmeas	0.010	0.083
Number of reflections	138026	21954
<I/σ(I)>	9.57	1.59
Completeness [%]	99.0	97.5
Redundancy	3.51
CC(1/2)	0.997	0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1M succinic acid pH 7.0, 0.1 M HEPES pH 7.0, 1 %(w/v) PEG 2000 MME