6F77

Crystal structure of the prephenate aminotransferase from Rhizobium meliloti

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-19
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979310
Spacegroup name	P 1 21 1
Unit cell lengths	103.543, 93.008, 123.069
Unit cell angles	90.00, 91.36, 90.00

Refinement procedure

Resolution	48.118 - 1.794
R-factor	0.1761
Rwork	0.174
R-free	0.20870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f5v
RMSD bond length	0.007
RMSD bond angle	0.876
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.118	1.900
High resolution limit [Å]	1.790	1.790
Number of reflections	213629	33152
<I/σ(I)>	9.77	2.28
Completeness [%]	98.0	94.4
Redundancy	2.96	2.94
CC(1/2)	0.995	0.729

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2 M ammonium formate, 19 % PEG 3350