6F75
Carboxypeptidase T mutant L254N with Sulphamoil Leucine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-04 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.8 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 158.930, 158.930, 105.400 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.120 - 1.890 |
R-factor | 0.15744 |
Rwork | 0.156 |
R-free | 0.18114 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.385 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.120 | 1.939 |
High resolution limit [Å] | 1.890 | 1.890 |
Number of reflections | 59832 | |
<I/σ(I)> | 4.2538 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 296 | 1,4 SA |