6F74
Crystal structure of VAO-type flavoprotein MtVAO713 from Myceliophthora thermophila C1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-11-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.574 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 83.195, 108.506, 135.989 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 54.250 - 2.200 |
| R-factor | 0.19793 |
| Rwork | 0.196 |
| R-free | 0.23859 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | MtVAO615 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.575 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.300 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.148 | 0.483 |
| Rpim | 0.114 | 0.479 |
| Number of reflections | 120921 | 5895 |
| <I/σ(I)> | 3.9 | 1.6 |
| Completeness [%] | 98.8 | 97.3 |
| Redundancy | 2.1 | 2.1 |
| CC(1/2) | 0.968 | 0.532 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 25% PEG3350, 0.1 M Tris pH 8.5-9.0 |
