6F72

Crystal structure of VAO-type flavoprotein MtVAO615 at pH 7.5 from Myceliophthora thermophila C1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	BRUKER AXS MICROSTAR
Temperature [K]	110
Detector technology	IMAGE PLATE
Collection date	2014-11-29
Detector	MARRESEARCH
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	59.700, 100.930, 111.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	51.400 - 2.000
R-factor	0.23786
Rwork	0.236
R-free	0.28044
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	D_1200007842
RMSD bond length	0.010
RMSD bond angle	1.444
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.400	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.172	0.986
Rpim	0.114	0.707
Number of reflections	45720	2754
<I/σ(I)>	4.7	1
Completeness [%]	98.1	81.1
Redundancy	3.7	3.2
CC(1/2)	0.988	0.333

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	11% PEG3350, 20% glycerol, 0.1 M HEPES pH 7.5