6F6O

Structure of Adenovirus 3 fiber head V239D mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-15
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	0.873
Spacegroup name	P 21 3
Unit cell lengths	81.867, 81.867, 81.867
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.890 - 1.490
R-factor	0.15228
Rwork	0.151
R-free	0.16984
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wyj
RMSD bond length	0.018
RMSD bond angle	1.818
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	81.800	1.580
High resolution limit [Å]	1.490	1.490
Rmeas	0.040	0.329
Number of reflections	27755	3062
<I/σ(I)>	23.6	2.8
Completeness [%]	92.2	63.9
Redundancy	4.6
CC(1/2)	0.990	0.870

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	15% PEG3350, HEPES, pH 7.4