6F63

Crystal structure of the SYCP1 C-terminal back-to-back assembly

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-19
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	233.420, 42.850, 43.690
Unit cell angles	90.00, 93.61, 90.00

Refinement procedure

Resolution	58.239 - 2.154
R-factor	0.2203
Rwork	0.219
R-free	0.25260
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.511
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	Arcimboldo
Refinement software	PHENIX ((1.12rc1_2807: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	116.480	2.270
High resolution limit [Å]	2.150	2.150
Rmerge	0.052	0.695
Rmeas	0.061	0.821
Rpim	0.032	0.429
Number of reflections	23092	3024
<I/σ(I)>	12.4	1.9
Completeness [%]	97.4	88
Redundancy	3.6
CC(1/2)	0.998	0.872

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	3.5 M sodium formate pH 7.0; soaked in 6 M sodium formate pH 7.0