6F63
Crystal structure of the SYCP1 C-terminal back-to-back assembly
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-02-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 233.420, 42.850, 43.690 |
| Unit cell angles | 90.00, 93.61, 90.00 |
Refinement procedure
| Resolution | 58.239 - 2.154 |
| R-factor | 0.2203 |
| Rwork | 0.219 |
| R-free | 0.25260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.511 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | Arcimboldo |
| Refinement software | PHENIX ((1.12rc1_2807: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 116.480 | 2.270 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.052 | 0.695 |
| Rmeas | 0.061 | 0.821 |
| Rpim | 0.032 | 0.429 |
| Number of reflections | 23092 | 3024 |
| <I/σ(I)> | 12.4 | 1.9 |
| Completeness [%] | 97.4 | 88 |
| Redundancy | 3.6 | |
| CC(1/2) | 0.998 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 3.5 M sodium formate pH 7.0; soaked in 6 M sodium formate pH 7.0 |
