6F62

Crystal structure of the SYCP1 N-terminal head-to-head assembly in open conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-08
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	I 1 2 1
Unit cell lengths	65.670, 37.310, 108.520
Unit cell angles	90.00, 106.66, 90.00

Refinement procedure

Resolution	27.234 - 2.066
R-factor	0.2274
Rwork	0.226
R-free	0.24410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.334
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	AMPLE
Refinement software	PHENIX ((1.12rc1_2807: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.870	2.120
High resolution limit [Å]	2.060	2.060
Rmerge	0.071	0.919
Rmeas	0.075	0.963
Rpim	0.023	0.286
Number of reflections	15862	1217
<I/σ(I)>	15	1.8
Completeness [%]	99.9	100
Redundancy	11	11.4
CC(1/2)	0.999	0.969

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	100 mM MES pH 6.0, 40% (v/v) MPD