6F4O

Human JMJD5 in complex with Mn(II) and Succinate.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	49.434, 64.411, 77.491
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.560 - 1.280
R-factor	0.147
Rwork	0.146
R-free	0.17440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gjz
RMSD bond length	0.011
RMSD bond angle	1.121
Data reduction software	HKL-2000 (706E)
Data scaling software	HKL-2000 (706E)
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	27.560	1.330
High resolution limit [Å]	1.280	1.280
Rmerge	0.081	1.100
Rpim	0.035	0.491
Number of reflections	64327	6378
<I/σ(I)>	19.5	1.9
Completeness [%]	99.7	100
Redundancy	7.1	7.4
CC(1/2)	0.996	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1 M Bis-Tris pH 6.5, 15.0 % PEG3350, 0.002 M MnCl2, 300 nl sitting drops (sample:well, 2:1 ratio)