6F4N

Human JMJD5 in complex with MN and 2OG.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-01-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 32 2 1
Unit cell lengths	68.002, 68.002, 267.898
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.160 - 2.541
R-factor	0.2009
Rwork	0.199
R-free	0.22990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gjz
RMSD bond length	0.005
RMSD bond angle	0.877
Data reduction software	HKL-2000 (706E)
Data scaling software	HKL-2000 (706E)
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.160	2.630
High resolution limit [Å]	2.540	2.540
Rmerge	0.081	1.100
Rpim	0.030	0.423
Number of reflections	24683	2411
<I/σ(I)>	25.1	1.8
Completeness [%]	99.9	99.7
Redundancy	9.4	8.7
CC(1/2)	0.999	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	0.1 M Bis-Tris pH 6.0, 21.4 % PEG3350, 0.07 M MgCl2, 0.002 M MnCl2, 300 nl sitting drops (sample:well, 1:2 ratio)