6F4M

Human JMJD5 in its apo form.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	49.033, 66.438, 70.076
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.210 - 1.705
R-factor	0.1705
Rwork	0.170
R-free	0.18650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4gjz
RMSD bond length	0.005
RMSD bond angle	0.764
Data reduction software	HKL-2000 (706E)
Data scaling software	HKL-2000 (706E)
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.210	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.101	1.600
Rpim	0.040	0.791
Number of reflections	25416	2481
<I/σ(I)>	21.6	1.9
Completeness [%]	99.8	99.7
Redundancy	12.6	13
CC(1/2)	0.998	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	298	0.1 M Bis-Tris pH 6.5, 22.5 % PEG3350, 0.002 M MnCl2, 300 nl sitting drops (sample:well, 1:1 ratio)