6F4C
Nicotiana benthamiana alpha-galactosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-07 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 74.037, 74.037, 133.307 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.720 - 2.800 |
| R-factor | 0.21432 |
| Rwork | 0.211 |
| R-free | 0.28505 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uas |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.545 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.720 | 64.720 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 9569 | |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 98.8 | |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 298 | 0.2 M LiSO4 0.1 M BIS-TRIS 5.5 pH 25 %w/v PEG 3350 |
