6F4B
Crystal structure of Glutathione Transferase Omega 3S from Trametes versicolor in complex with glutathione
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.980 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.470, 104.180, 107.920 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.914 - 1.550 |
R-factor | 0.1611 |
Rwork | 0.160 |
R-free | 0.18180 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6f43 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.260 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.920 | 1.590 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.062 | |
Number of reflections | 82933 | |
<I/σ(I)> | 22.2 | |
Completeness [%] | 99.5 | |
Redundancy | 7.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 278 | 30 % PEG 400 0.2 M calcium acetate 0.1 M acetate buffer, pH 4.5 |