6F4A

Yeast mitochondrial RNA degradosome complex mtEXO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-08
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97625
Spacegroup name	C 2 2 21
Unit cell lengths	104.550, 151.240, 284.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.061 - 3.550
R-factor	0.2978
Rwork	0.296
R-free	0.32790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6f3h 3rc3
RMSD bond length	0.007
RMSD bond angle	1.440
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.100	3.640
High resolution limit [Å]	3.550	3.550
Rmeas	0.033	0.225
Number of reflections	26391
<I/σ(I)>	27.4
Completeness [%]	95.4
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M ammonium citrate tribasic (pH 7.0), 18% (w/v) PEG3350