6F49
Periplasmic domain of LolC lacking the Hook.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-08 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 75.310, 108.540, 109.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.040 - 2.020 |
| R-factor | 0.19061 |
| Rwork | 0.188 |
| R-free | 0.23279 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5naa |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.704 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.040 | 2.070 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Number of reflections | 58914 | 4321 |
| <I/σ(I)> | 6.7 | 2 |
| Completeness [%] | 99.1 | 99.5 |
| Redundancy | 5 | 5.2 |
| CC(1/2) | 0.704 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 288.15 | 0.2 M ammonium sulfate, 0.15 M sodium acetate pH 4.6, 30% w/v PEG 2000 MME |
