6F45

Crystal structure of the gp37-gp38 adhesin tip complex of the bacteriophage S16 long tail fiber

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	C 2 2 21
Unit cell lengths	81.337, 98.904, 140.036
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.760 - 1.704
R-factor	0.15333184313
Rwork	0.152
R-free	0.18463
Structure solution method	SAD
RMSD bond length	0.016
RMSD bond angle	1.378
Data reduction software	XDS (Version 07/17/15)
Data scaling software	SCALA (1.10.28)
Phasing software	PARROT (1.0.3)
Refinement software	PHENIX (1.10_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.760	1.810
High resolution limit [Å]	1.700	1.700
Rmerge	0.065	0.729
Rmeas	0.068	0.759
Rpim	0.019	0.208
Number of reflections	118554	18499
<I/σ(I)>	16.66	2.36
Completeness [%]	99.3	95.9
Redundancy	6.86	6.69
CC(1/2)	0.990	0.929

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	292	35% (v/v) Hexylene Glycol, 200 mM Magnesium Chloride, 100 mM Imidazole, pH 8.0