6F3Z

Complex of E. coli LolA and periplasmic domain of LolC

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-08
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 2
Unit cell lengths	146.010, 68.230, 94.780
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	73.010 - 2.000
R-factor	0.2046
Rwork	0.202
R-free	0.24920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5naa 1iwl
RMSD bond length	0.019
RMSD bond angle	1.854
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.010	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.103
Number of reflections	61745
<I/σ(I)>	10.6	2.2
Completeness [%]	95.8	97
Redundancy	10.2	9.9
CC(1/2)		0.883

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		288.15	45% w/v Poly(acrylic acid sodium salt) 2100, 100 mM HEPES pH 6.5